Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCR

BACKGROUND: Diagnosis of enterotoxigenic E. coli (ETEC) associated diarrhea is complicated by the diversity of E.coli virulence factors. This study developed a multiplex quantitative PCR assay based on high-resolution melting curves analysis (HRM-qPCR) to identify and quantify genes encoding five ET...

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Detalles Bibliográficos
Autores principales: Wang, Weilan, Zijlstra, Ruurd T., Gänzle, Michael G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433089/
https://www.ncbi.nlm.nih.gov/pubmed/28506262
http://dx.doi.org/10.1186/s12866-017-1023-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433089/
https://www.ncbi.nlm.nih.gov/pubmed/28506262
http://dx.doi.org/10.1186/s12866-017-1023-5

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