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Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research

Vast numbers of proteins work cooperatively to exert their functions in various cells. In order to understand the functions and molecular mechanisms of these proteins in plants, analyses of transgenic plants that concomitantly express two protein-coding genes are often required. We developed a novel...

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Detalles Bibliográficos
Autores principales: Aboulela, Mostafa, Tanaka, Yuji, Nishimura, Kohji, Mano, Shoji, Nishimura, Mikio, Ishiguro, Sumie, Kimura, Tetsuya, Nakagawa, Tsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433782/
https://www.ncbi.nlm.nih.gov/pubmed/28520787
http://dx.doi.org/10.1371/journal.pone.0177889