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Development of an R4 dual-site (R4DS) gateway cloning system enabling the efficient simultaneous cloning of two desired sets of promoters and open reading frames in a binary vector for plant research
Vast numbers of proteins work cooperatively to exert their functions in various cells. In order to understand the functions and molecular mechanisms of these proteins in plants, analyses of transgenic plants that concomitantly express two protein-coding genes are often required. We developed a novel...
Autores principales: | Aboulela, Mostafa, Tanaka, Yuji, Nishimura, Kohji, Mano, Shoji, Nishimura, Mikio, Ishiguro, Sumie, Kimura, Tetsuya, Nakagawa, Tsuyoshi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433782/ https://www.ncbi.nlm.nih.gov/pubmed/28520787 http://dx.doi.org/10.1371/journal.pone.0177889 |
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