Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy

The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation. A variety of ligase-independent PCR cloning st...

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Detalles Bibliográficos
Autores principales: Cao, Liting, Zhou, Yancheng, Huang, Lin, Dong, Shiqi, Ma, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433943/
https://www.ncbi.nlm.nih.gov/pubmed/28510221
http://dx.doi.org/10.1186/s13568-017-0386-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433943/
https://www.ncbi.nlm.nih.gov/pubmed/28510221
http://dx.doi.org/10.1186/s13568-017-0386-1

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