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Development of a dual-expression vector facilitated with selection-free PCR recombination cloning strategy
The conventional procedure for the construction of recombinant expression vector of a target gene includes PCR cloning and restriction enzyme mediated subcloning, which is time-consuming and sometimes troublesome because of the inefficiency of ligation. A variety of ligase-independent PCR cloning st...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Berlin Heidelberg
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5433943/ https://www.ncbi.nlm.nih.gov/pubmed/28510221 http://dx.doi.org/10.1186/s13568-017-0386-1 |