Enhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli

BACKGROUND: Human Cu/Zn superoxide dismutase (hSOD1) is an antioxidant enzyme with potential as a therapeutic agent. However, heterologous expression of hSOD1 has remained an issue due to Cu(2+) insufficiency at protein active site, leading to low solubility and enzymatic activity. OBJECTIVES: The e...

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Detalles Bibliográficos
Autores principales: Eiamphungporn, Warawan, Yainoy, Sakda, Prachayasittikul, Virapong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Institute of Genetic Engineering and Biotechnology 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5434994/
https://www.ncbi.nlm.nih.gov/pubmed/28959342
http://dx.doi.org/10.15171/ijb.1465

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5434994/
https://www.ncbi.nlm.nih.gov/pubmed/28959342
http://dx.doi.org/10.15171/ijb.1465

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