Validation of reference genes for the normalization of the RT-qPCR gene expression of virulence genes of Erwinia amylovora in apple shoots

To study the expression of pathogenicity-related genes in Erwinia amylovora, seven candidate reference genes (ffh, glyA, gyrA, proC, pykA, recA, rpoB) were selected and validated with the following five different mathematic algorithms: geNorm, NormFinder, BestKeeper, the delta CT method and the RefF...

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Detalles Bibliográficos
Autores principales: Kałużna, Monika, Kuras, Anita, Puławska, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435713/
https://www.ncbi.nlm.nih.gov/pubmed/28515453
http://dx.doi.org/10.1038/s41598-017-02078-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435713/
https://www.ncbi.nlm.nih.gov/pubmed/28515453
http://dx.doi.org/10.1038/s41598-017-02078-4

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