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No Functional Role for microRNA-342 in a Mouse Model of Pancreatic Acinar Carcinoma

The intronic microRNA (miR)-342 has been proposed as a potent tumor-suppressor gene. miR-342 is found to be downregulated or epigenetically silenced in multiple different tumor sites, and this loss of expression permits the upregulation of several key oncogenic pathways. In several different cell li...

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Autores principales: Dooley, James, Lagou, Vasiliki, Pasciuto, Emanuela, Linterman, Michelle A., Prosser, Haydn M., Himmelreich, Uwe, Liston, Adrian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435746/
https://www.ncbi.nlm.nih.gov/pubmed/28573106
http://dx.doi.org/10.3389/fonc.2017.00101
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author Dooley, James
Lagou, Vasiliki
Pasciuto, Emanuela
Linterman, Michelle A.
Prosser, Haydn M.
Himmelreich, Uwe
Liston, Adrian
author_facet Dooley, James
Lagou, Vasiliki
Pasciuto, Emanuela
Linterman, Michelle A.
Prosser, Haydn M.
Himmelreich, Uwe
Liston, Adrian
author_sort Dooley, James
collection PubMed
description The intronic microRNA (miR)-342 has been proposed as a potent tumor-suppressor gene. miR-342 is found to be downregulated or epigenetically silenced in multiple different tumor sites, and this loss of expression permits the upregulation of several key oncogenic pathways. In several different cell lines, lower miR-342 expression results in enhanced proliferation and metastasis potential, both in vitro and in xenogenic transplant conditions. Here, we sought to determine the function of miR-342 in an in vivo spontaneous cancer model, using the Ela1-TAg transgenic model of pancreatic acinar carcinoma. Through longitudinal magnetic resonance imaging monitoring of Ela1-TAg transgenic mice, either wild-type or knockout for miR-342, we found no role for miR-342 in the development, growth rate, or pathogenicity of pancreatic acinar carcinoma. These results indicate the importance of assessing miR function in the complex physiology of in vivo model systems and indicate that further functional testing of miR-342 is required before concluding it is a bona fide tumor-suppressor-miR.
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spelling pubmed-54357462017-06-01 No Functional Role for microRNA-342 in a Mouse Model of Pancreatic Acinar Carcinoma Dooley, James Lagou, Vasiliki Pasciuto, Emanuela Linterman, Michelle A. Prosser, Haydn M. Himmelreich, Uwe Liston, Adrian Front Oncol Oncology The intronic microRNA (miR)-342 has been proposed as a potent tumor-suppressor gene. miR-342 is found to be downregulated or epigenetically silenced in multiple different tumor sites, and this loss of expression permits the upregulation of several key oncogenic pathways. In several different cell lines, lower miR-342 expression results in enhanced proliferation and metastasis potential, both in vitro and in xenogenic transplant conditions. Here, we sought to determine the function of miR-342 in an in vivo spontaneous cancer model, using the Ela1-TAg transgenic model of pancreatic acinar carcinoma. Through longitudinal magnetic resonance imaging monitoring of Ela1-TAg transgenic mice, either wild-type or knockout for miR-342, we found no role for miR-342 in the development, growth rate, or pathogenicity of pancreatic acinar carcinoma. These results indicate the importance of assessing miR function in the complex physiology of in vivo model systems and indicate that further functional testing of miR-342 is required before concluding it is a bona fide tumor-suppressor-miR. Frontiers Media S.A. 2017-05-18 /pmc/articles/PMC5435746/ /pubmed/28573106 http://dx.doi.org/10.3389/fonc.2017.00101 Text en Copyright © 2017 Dooley, Lagou, Pasciuto, Linterman, Prosser, Himmelreich and Liston. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Dooley, James
Lagou, Vasiliki
Pasciuto, Emanuela
Linterman, Michelle A.
Prosser, Haydn M.
Himmelreich, Uwe
Liston, Adrian
No Functional Role for microRNA-342 in a Mouse Model of Pancreatic Acinar Carcinoma
title No Functional Role for microRNA-342 in a Mouse Model of Pancreatic Acinar Carcinoma
title_full No Functional Role for microRNA-342 in a Mouse Model of Pancreatic Acinar Carcinoma
title_fullStr No Functional Role for microRNA-342 in a Mouse Model of Pancreatic Acinar Carcinoma
title_full_unstemmed No Functional Role for microRNA-342 in a Mouse Model of Pancreatic Acinar Carcinoma
title_short No Functional Role for microRNA-342 in a Mouse Model of Pancreatic Acinar Carcinoma
title_sort no functional role for microrna-342 in a mouse model of pancreatic acinar carcinoma
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435746/
https://www.ncbi.nlm.nih.gov/pubmed/28573106
http://dx.doi.org/10.3389/fonc.2017.00101
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