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3′ Uridylation controls mature microRNA turnover during CD4 T-cell activation

Activation of T lymphocytes requires a tight regulation of microRNA (miRNA) expression. Terminal uridyltransferases (TUTases) catalyze 3′ nontemplated nucleotide addition (3′NTA) to miRNAs, which may influence miRNA stability and function. Here, we investigated 3′NTA to mature miRNA in CD4 T lymphoc...

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Autores principales: Gutiérrez-Vázquez, Cristina, Enright, Anton J., Rodríguez-Galán, Ana, Pérez-García, Arantxa, Collier, Paul, Jones, Matthew R., Benes, Vladimir, Mizgerd, Joseph P., Mittelbrunn, María, Ramiro, Almudena R., Sánchez-Madrid, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435861/
https://www.ncbi.nlm.nih.gov/pubmed/28351886
http://dx.doi.org/10.1261/rna.060095.116
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author Gutiérrez-Vázquez, Cristina
Enright, Anton J.
Rodríguez-Galán, Ana
Pérez-García, Arantxa
Collier, Paul
Jones, Matthew R.
Benes, Vladimir
Mizgerd, Joseph P.
Mittelbrunn, María
Ramiro, Almudena R.
Sánchez-Madrid, Francisco
author_facet Gutiérrez-Vázquez, Cristina
Enright, Anton J.
Rodríguez-Galán, Ana
Pérez-García, Arantxa
Collier, Paul
Jones, Matthew R.
Benes, Vladimir
Mizgerd, Joseph P.
Mittelbrunn, María
Ramiro, Almudena R.
Sánchez-Madrid, Francisco
author_sort Gutiérrez-Vázquez, Cristina
collection PubMed
description Activation of T lymphocytes requires a tight regulation of microRNA (miRNA) expression. Terminal uridyltransferases (TUTases) catalyze 3′ nontemplated nucleotide addition (3′NTA) to miRNAs, which may influence miRNA stability and function. Here, we investigated 3′NTA to mature miRNA in CD4 T lymphocytes by deep sequencing. Upon T-cell activation, miRNA sequences bearing terminal uridines are specifically decreased, concomitantly with down-regulation of TUT4 and TUT7 enzymes. Analyzing TUT4-deficient T lymphocytes, we proved that this terminal uridyltransferase is essential for the maintenance of miRNA uridylation in the steady state of T lymphocytes. Analysis of synthetic uridylated miRNAs shows that 3′ addition of uridine promotes degradation of these uridylated miRNAs after T-cell activation. Our data underline post-transcriptional uridylation as a mechanism to fine-tune miRNA levels during T-cell activation.
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spelling pubmed-54358612018-06-01 3′ Uridylation controls mature microRNA turnover during CD4 T-cell activation Gutiérrez-Vázquez, Cristina Enright, Anton J. Rodríguez-Galán, Ana Pérez-García, Arantxa Collier, Paul Jones, Matthew R. Benes, Vladimir Mizgerd, Joseph P. Mittelbrunn, María Ramiro, Almudena R. Sánchez-Madrid, Francisco RNA Report Activation of T lymphocytes requires a tight regulation of microRNA (miRNA) expression. Terminal uridyltransferases (TUTases) catalyze 3′ nontemplated nucleotide addition (3′NTA) to miRNAs, which may influence miRNA stability and function. Here, we investigated 3′NTA to mature miRNA in CD4 T lymphocytes by deep sequencing. Upon T-cell activation, miRNA sequences bearing terminal uridines are specifically decreased, concomitantly with down-regulation of TUT4 and TUT7 enzymes. Analyzing TUT4-deficient T lymphocytes, we proved that this terminal uridyltransferase is essential for the maintenance of miRNA uridylation in the steady state of T lymphocytes. Analysis of synthetic uridylated miRNAs shows that 3′ addition of uridine promotes degradation of these uridylated miRNAs after T-cell activation. Our data underline post-transcriptional uridylation as a mechanism to fine-tune miRNA levels during T-cell activation. Cold Spring Harbor Laboratory Press 2017-06 /pmc/articles/PMC5435861/ /pubmed/28351886 http://dx.doi.org/10.1261/rna.060095.116 Text en © 2017 Gutiérrez-Vázquez et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Report
Gutiérrez-Vázquez, Cristina
Enright, Anton J.
Rodríguez-Galán, Ana
Pérez-García, Arantxa
Collier, Paul
Jones, Matthew R.
Benes, Vladimir
Mizgerd, Joseph P.
Mittelbrunn, María
Ramiro, Almudena R.
Sánchez-Madrid, Francisco
3′ Uridylation controls mature microRNA turnover during CD4 T-cell activation
title 3′ Uridylation controls mature microRNA turnover during CD4 T-cell activation
title_full 3′ Uridylation controls mature microRNA turnover during CD4 T-cell activation
title_fullStr 3′ Uridylation controls mature microRNA turnover during CD4 T-cell activation
title_full_unstemmed 3′ Uridylation controls mature microRNA turnover during CD4 T-cell activation
title_short 3′ Uridylation controls mature microRNA turnover during CD4 T-cell activation
title_sort 3′ uridylation controls mature microrna turnover during cd4 t-cell activation
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5435861/
https://www.ncbi.nlm.nih.gov/pubmed/28351886
http://dx.doi.org/10.1261/rna.060095.116
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