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Optimizing the Parameters Governing the Fragmentation of Cross-Linked Peptides in a Tribrid Mass Spectrometer

[Image: see text] We compared the five different ways of fragmentation available on a tribrid mass spectrometer and optimized their collision energies with regard to optimal sequence coverage of cross-linked peptides. We created a library of bis(sulfosuccinimidyl)suberate (BS3/DSS) cross-linked prec...

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Detalles Bibliográficos
Autores principales: Kolbowski, Lars, Mendes, Marta L., Rappsilber, Juri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5436099/
https://www.ncbi.nlm.nih.gov/pubmed/28402676
http://dx.doi.org/10.1021/acs.analchem.6b04935