Precise genome-wide base editing by the CRISPR Nickase system in yeast

The CRISPR/Cas9 system has been applied to efficient genome editing in many eukaryotic cells. However, the bases that can be edited by this system have been limited to those within the protospacer adjacent motif (PAM) and guide RNA-targeting sequences. In this study, we developed a genome-wide base...

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Detalles Bibliográficos
Autores principales: Satomura, Atsushi, Nishioka, Ryosuke, Mori, Hitoshi, Sato, Kosuke, Kuroda, Kouichi, Ueda, Mitsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437071/
https://www.ncbi.nlm.nih.gov/pubmed/28522803
http://dx.doi.org/10.1038/s41598-017-02013-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437071/
https://www.ncbi.nlm.nih.gov/pubmed/28522803
http://dx.doi.org/10.1038/s41598-017-02013-7

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