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A rapid and low-cost estimation of bacteria counts in solution using fluorescence spectroscopy

The fluorescence spectrum of bacterially bound acridine orange (AO) was investigated to evaluate its use for the rapid enumeration of bacteria. Escherichia coli ATCC 25922 samples were stained with 2 × 10(−2), 2 × 10(−3) or 2 × 10(−4)% w/v AO, followed by 3, 2 or 0 washing cycles, respectively, and...

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Detalles Bibliográficos
Autores principales: Guo, Rachel, McGoverin, Cushla, Swift, Simon, Vanholsbeeck, Frederique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437196/
https://www.ncbi.nlm.nih.gov/pubmed/28389919
http://dx.doi.org/10.1007/s00216-017-0347-1