Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification

Alternative messenger RNA (mRNA) splicing is a basic mechanism of gene regulation. In general, reverse transcription and polymerase based primer extension limit the sensitivity and selectivity of the current detection of mRNA splice variants, respectively. Here, we show that, using the ligation of t...

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Detalles Bibliográficos
Autores principales: Wang, Honghong, Wang, Hui, Duan, Xinrui, Sun, Yuanyuan, Wang, Xiangdong, Li, Zhengping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437374/
https://www.ncbi.nlm.nih.gov/pubmed/28580102
http://dx.doi.org/10.1039/c7sc00094d

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5437374/
https://www.ncbi.nlm.nih.gov/pubmed/28580102
http://dx.doi.org/10.1039/c7sc00094d

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