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Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector

Cloning of multiple genes in a single vector has greatly facilitated both basic and translational studies that require co-expression of multiple factors or multi-units of complex protein. Many strategies have been adopted, among which 2A “self-cleaving” peptides have garnered increased interest for...

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Detalles Bibliográficos
Autores principales: Liu, Ziqing, Chen, Olivia, Wall, J. Blake Joseph, Zheng, Michael, Zhou, Yang, Wang, Li, Ruth Vaseghi, Haley, Qian, Li, Liu, Jiandong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438344/
https://www.ncbi.nlm.nih.gov/pubmed/28526819
http://dx.doi.org/10.1038/s41598-017-02460-2