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Cell fixation and preservation for droplet-based single-cell transcriptomics

BACKGROUND: Recent developments in droplet-based microfluidics allow the transcriptional profiling of thousands of individual cells in a quantitative, highly parallel and cost-effective way. A critical, often limiting step is the preparation of cells in an unperturbed state, not altered by stress or...

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Detalles Bibliográficos
Autores principales: Alles, Jonathan, Karaiskos, Nikos, Praktiknjo, Samantha D., Grosswendt, Stefanie, Wahle, Philipp, Ruffault, Pierre-Louis, Ayoub, Salah, Schreyer, Luisa, Boltengagen, Anastasiya, Birchmeier, Carmen, Zinzen, Robert, Kocks, Christine, Rajewsky, Nikolaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5438562/
https://www.ncbi.nlm.nih.gov/pubmed/28526029
http://dx.doi.org/10.1186/s12915-017-0383-5