Thermal stability and kinetic constants for 129 variants of a family 1 glycoside hydrolase reveal that enzyme activity and stability can be separately designed

Accurate modeling of enzyme activity and stability is an important goal of the protein engineering community. However, studies seeking to evaluate current progress are limited by small data sets of quantitative kinetic constants and thermal stability measurements. Here, we report quantitative measur...

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Detalles Bibliográficos
Autores principales: Carlin, Dylan Alexander, Hapig-Ward, Siena, Chan, Bill Wayne, Damrau, Natalie, Riley, Mary, Caster, Ryan W., Bethards, Bowen, Siegel, Justin B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5439667/
https://www.ncbi.nlm.nih.gov/pubmed/28531185
http://dx.doi.org/10.1371/journal.pone.0176255

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5439667/
https://www.ncbi.nlm.nih.gov/pubmed/28531185
http://dx.doi.org/10.1371/journal.pone.0176255

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