Differentially expressed genes from RNA-Seq and functional enrichment results are affected by the choice of single-end versus paired-end reads and stranded versus non-stranded protocols

BACKGROUND: RNA-Seq is now widely used as a research tool. Choices must be made whether to use paired-end (PE) or single-end (SE) sequencing, and whether to use strand-specific or non-specific (NS) library preparation kits. To date there has been no analysis of the effect of these choices on identif...

Descripción completa

Detalles Bibliográficos
Autores principales: Corley, Susan M., MacKenzie, Karen L., Beverdam, Annemiek, Roddam, Louise F., Wilkins, Marc R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442695/
https://www.ncbi.nlm.nih.gov/pubmed/28535780
http://dx.doi.org/10.1186/s12864-017-3797-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5442695/
https://www.ncbi.nlm.nih.gov/pubmed/28535780
http://dx.doi.org/10.1186/s12864-017-3797-0

Ejemplares similares