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Chemical and structural characterization of a model Post-Termination Complex (PoTC) for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-G

A model Post-Termination Complex (PoTC) used for the discovery of Ribosome Recycling Factor (RRF) was purified and characterized by cryo-electron microscopic analysis and biochemical methods. We established that the model PoTC has mostly one tRNA, at the P/E or P/P position, together with one mRNA....

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Detalles Bibliográficos
Autores principales: Iwakura, Nobuhiro, Yokoyama, Takeshi, Quaglia, Fabio, Mitsuoka, Kaoru, Mio, Kazuhiro, Shigematsu, Hideki, Shirouzu, Mikako, Kaji, Akira, Kaji, Hideko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443523/
https://www.ncbi.nlm.nih.gov/pubmed/28542628
http://dx.doi.org/10.1371/journal.pone.0177972