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Chemical and structural characterization of a model Post-Termination Complex (PoTC) for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-G
A model Post-Termination Complex (PoTC) used for the discovery of Ribosome Recycling Factor (RRF) was purified and characterized by cryo-electron microscopic analysis and biochemical methods. We established that the model PoTC has mostly one tRNA, at the P/E or P/P position, together with one mRNA....
Autores principales: | Iwakura, Nobuhiro, Yokoyama, Takeshi, Quaglia, Fabio, Mitsuoka, Kaoru, Mio, Kazuhiro, Shigematsu, Hideki, Shirouzu, Mikako, Kaji, Akira, Kaji, Hideko |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5443523/ https://www.ncbi.nlm.nih.gov/pubmed/28542628 http://dx.doi.org/10.1371/journal.pone.0177972 |
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