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A Split Staphylococcus aureus Cas9 as a Compact Genome-Editing Tool in Plants

Split-protein methods—where a protein is split into two inactive fragments that must re-assemble to form an active protein—can be used to regulate the activity of a given protein and reduce the size of gene transcription units. Here, we show that a Staphylococcus aureus Cas9 (SaCas9) can be split, a...

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Detalles Bibliográficos
Autores principales: Kaya, Hidetaka, Ishibashi, Kazuhiro, Toki, Seiichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5444561/
https://www.ncbi.nlm.nih.gov/pubmed/28371831
http://dx.doi.org/10.1093/pcp/pcx034