Reducing mitochondrial reads in ATAC-seq using CRISPR/Cas9

ATAC-seq is a high-throughput sequencing technique that identifies open chromatin. Depending on the cell type, ATAC-seq samples may contain ~20–80% of mitochondrial sequencing reads. As the regions of open chromatin of interest are usually located in the nuclear genome, mitochondrial reads are typic...

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Detalles Bibliográficos
Autores principales: Montefiori, Lindsey, Hernandez, Liana, Zhang, Zijie, Gilad, Yoav, Ober, Carole, Crawford, Gregory, Nobrega, Marcelo, Jo Sakabe, Noboru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446398/
https://www.ncbi.nlm.nih.gov/pubmed/28550296
http://dx.doi.org/10.1038/s41598-017-02547-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446398/
https://www.ncbi.nlm.nih.gov/pubmed/28550296
http://dx.doi.org/10.1038/s41598-017-02547-w

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