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Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation
BACKGROUND: Antibody fragments are molecules widely used for diagnosis and therapy. A large amount of protein is frequently required for such applications. New approaches using folding reporter enzymes have recently been proposed to increase soluble expression of foreign proteins in Escherichia coli...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2004
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC544847/ https://www.ncbi.nlm.nih.gov/pubmed/15606918 http://dx.doi.org/10.1186/1475-2859-3-16 |