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Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression

BACKGROUND: In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein. Proteins studied included cell surface receptors (Ephrins and Eph receptors, CD44), kinases (EGFR...

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Detalles Bibliográficos
Autores principales: Dyson, Michael R, Shadbolt, S Paul, Vincent, Karen J, Perera, Rajika L, McCafferty, John
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC544853/
https://www.ncbi.nlm.nih.gov/pubmed/15598350
http://dx.doi.org/10.1186/1472-6750-4-32