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Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression
BACKGROUND: In the search for generic expression strategies for mammalian protein families several bacterial expression vectors were examined for their ability to promote high yields of soluble protein. Proteins studied included cell surface receptors (Ephrins and Eph receptors, CD44), kinases (EGFR...
Autores principales: | Dyson, Michael R, Shadbolt, S Paul, Vincent, Karen J, Perera, Rajika L, McCafferty, John |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC544853/ https://www.ncbi.nlm.nih.gov/pubmed/15598350 http://dx.doi.org/10.1186/1472-6750-4-32 |
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