Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice

Robust reproductive engineering techniques are required for the efficient and rapid production of genetically modified mice. We have reported the efficient production of genome-edited mice using reproductive engineering techniques, such as ultra-superovulation, in vitro fertilization (IVF) and vitri...

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Detalles Bibliográficos
Autores principales: Nakagawa, Yoshiko, Sakuma, Tetsushi, Nishimichi, Norihisa, Yokosaki, Yasuyuki, Takeo, Toru, Nakagata, Naomi, Yamamoto, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Methods & Techniques
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450330/
https://www.ncbi.nlm.nih.gov/pubmed/28396487
http://dx.doi.org/10.1242/bio.025122

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450330/
https://www.ncbi.nlm.nih.gov/pubmed/28396487
http://dx.doi.org/10.1242/bio.025122

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