Cargando…

Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice

Robust reproductive engineering techniques are required for the efficient and rapid production of genetically modified mice. We have reported the efficient production of genome-edited mice using reproductive engineering techniques, such as ultra-superovulation, in vitro fertilization (IVF) and vitri...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nakagawa, Yoshiko, Sakuma, Tetsushi, Nishimichi, Norihisa, Yokosaki, Yasuyuki, Takeo, Toru, Nakagata, Naomi, Yamamoto, Takashi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Company of Biologists Ltd 2017
Materias:	Methods & Techniques
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5450330/ https://www.ncbi.nlm.nih.gov/pubmed/28396487 http://dx.doi.org/10.1242/bio.025122

Ejemplares similares

Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice
por: Nakagawa, Yoshiko, et al.
Publicado: (2016)

Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation
por: Nakagawa, Yoshiko, et al.
Publicado: (2018)

Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote
por: Raveux, Aurélien, et al.
Publicado: (2017)

Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes
por: Nakagawa, Yoshiko, et al.
Publicado: (2015)

Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes
por: Crispo, M., et al.
Publicado: (2015)

New Therapeutic Targets for Hepatic Fibrosis in the Integrin Family, α8β1 and α11β1, Induced Specifically on Activated Stellate Cells
por: Yokosaki, Yasuyuki, et al.
Publicado: (2021)

N-acetyl cysteine restores the fertility of vitrified–warmed mouse oocytes derived through ultrasuperovulation
por: Mukunoki, Ayumi, et al.
Publicado: (2019)

Targeted gene knock-in by CRISPR/Cas ribonucleoproteins in porcine zygotes
por: Park, Ki-Eun, et al.
Publicado: (2017)

Epitopes in α8β1 and other RGD-binding integrins delineate classes of integrin-blocking antibodies and major binding loops in α subunits
por: Nishimichi, Norihisa, et al.
Publicado: (2015)

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes
por: Yoshimi, Kazuto, et al.
Publicado: (2016)

Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System
por: Zhang, Tao, et al.
Publicado: (2016)

Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ
por: Aida, Tomomi, et al.
Publicado: (2016)

Use of CRISPR/Cas9 technology efficiently targetted goat myostatin through zygotes microinjection resulting in double-muscled phenotype in goats
por: He, Zhengyi, et al.
Publicado: (2018)

Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice
por: Aida, Tomomi, et al.
Publicado: (2015)

One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes
por: Owen, Joseph R., et al.
Publicado: (2021)

Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN
por: Tanihara, Fuminori, et al.
Publicado: (2023)

Microinjection free delivery of miRNA inhibitor into zygotes
por: Joo, Jin Young, et al.
Publicado: (2014)

Chromosome engineering in zygotes with CRISPR/Cas9
por: Boroviak, Katharina, et al.
Publicado: (2016)

Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes
por: Chu, Van Trung, et al.
Publicado: (2016)

CRISPR/Cas9 microinjection in oocytes disables pancreas development in sheep
por: Vilarino, Marcela, et al.
Publicado: (2017)

Induced hepatic stellate cell integrin, α8β1, enhances cellular contractility and TGFβ activity in liver fibrosis
por: Nishimichi, Norihisa, et al.
Publicado: (2021)

Astaxanthin Supplementation Improves the Subsequent Developmental Competence of Vitrified Porcine Zygotes
por: Xiang, Decai, et al.
Publicado: (2022)

Electroporation and genetic supply of Cas9 increase the generation efficiency of CRISPR/Cas9 knock-in alleles in C57BL/6J mouse zygotes
por: Alghadban, Samy, et al.
Publicado: (2020)

The effect of CRISPR constructs microinjection on the expression of developmental genes in Rag1 knocked‐out mice embryo
por: Salimi, Maryam, et al.
Publicado: (2021)

Optimization of sand fly embryo microinjection for gene editing by CRISPR/Cas9
por: Martin-Martin, Ines, et al.
Publicado: (2018)

Genome-Wide Gene Expression Profiling Reveals the Direct Effect of Dienogest on Ovarian Endometriotic Stromal Cells
por: Honda, Hiroshi, et al.
Publicado: (2023)

Automated Microinjection of Recombinant BCL-X into Mouse Zygotes Enhances Embryo Development
por: Liu, Xinyu, et al.
Publicado: (2011)

Comparative Analysis of Genome Editors Efficiency on a Model of Mice Zygotes Microinjection
por: Averina, Olga A., et al.
Publicado: (2021)

Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering
por: Horii, Takuro, et al.
Publicado: (2014)

Author Correction: CRISPR/Cas9 microinjection in oocytes disables pancreas development in sheep
por: Vilarino, Marcela, et al.
Publicado: (2020)

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes
por: Liang, Puping, et al.
Publicado: (2015)

Pre-Vitrification and Post-Warming Variables of Vitrified-Warmed Blastocysts That Are Predictable for Implantation
por: Gabrielsen, Anette, et al.
Publicado: (2023)

Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos
por: TANIHARA, Fuminori, et al.
Publicado: (2019)

One-step generation of knockout pigs by zygote injection of CRISPR/Cas system
por: Hai, Tang, et al.
Publicado: (2014)

Efficient gene targeting in mouse zygotes mediated by CRISPR/Cas9-protein
por: Jung, Chris J., et al.
Publicado: (2016)

AANAT transgenic sheep generated via OPS vitrified-microinjected pronuclear embryos and reproduction efficiency of the transgenic offspring
por: Tian, Xiuzhi, et al.
Publicado: (2018)

CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells
por: Kimura, Yasuyoshi, et al.
Publicado: (2015)

Microinjection to deliver protein, mRNA, and DNA into zygotes of the cnidarian endosymbiosis model Aiptasia sp.
por: Jones, Victor A. S., et al.
Publicado: (2018)

Comparison of the effects of introducing the CRISPR/Cas9 system by microinjection and electroporation into porcine embryos at different stages
por: Le, Quynh Anh, et al.
Publicado: (2021)

Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing
por: Nakagawa, Yoshiko, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_unulklpn2dgk6f9l7pabcnve2e