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Decreased gene expression from T7 promoters may be due to impaired production of active T7 RNA polymerase
BACKGROUND: Protein expression vectors that utilize the bacteriophage T7 polymerase/promoter system are capable of very high levels of protein production. Frequently, however, expression from these vectors does not reliably achieve optimal levels of protein production. Strategies have been proposed...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC545050/ https://www.ncbi.nlm.nih.gov/pubmed/15638935 http://dx.doi.org/10.1186/1475-2859-4-3 |