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Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response

Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-α m RNA expression in monocytes. W...

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Autores principales: Zinkova, Alzbeta, Brynychova, Iva, Svacina, Alexander, Jirkovska, Marie, Korabecna, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453964/
https://www.ncbi.nlm.nih.gov/pubmed/28572683
http://dx.doi.org/10.1038/s41598-017-02905-8
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author Zinkova, Alzbeta
Brynychova, Iva
Svacina, Alexander
Jirkovska, Marie
Korabecna, Marie
author_facet Zinkova, Alzbeta
Brynychova, Iva
Svacina, Alexander
Jirkovska, Marie
Korabecna, Marie
author_sort Zinkova, Alzbeta
collection PubMed
description Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-α m RNA expression in monocytes. We performed qPCR to determine relative telomere length (T/S ratios) in plasma, serum and whole blood of 36 volunteers. Using paired samples of plasma and serum and DNase treatment, we analysed the contribution of cfDNA to the co-activation of TNF-α mRNA expression in THP1 monocytic cell line. We found significant differences between paired plasma and serum samples in relative T/S ratios (median 1.38 ± 1.1 vs. 0.86 ± 0.25, respectively) and in total amounts of cfDNA and in estimated total amounts of telomeres which were significantly higher in serum than in plasma. TNF-α mRNA expression in THP1 cells increased significantly after DNase treatment of all samples used for stimulation. The highest TNF-α mRNA expressions were observed after stimulation with DNase treated serum samples. Our results suggest that the different content of telomeric sequences in plasma and serum may contribute to the tuning of immune response. Further studies of this interesting phenomenon are needed.
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spelling pubmed-54539642017-06-02 Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response Zinkova, Alzbeta Brynychova, Iva Svacina, Alexander Jirkovska, Marie Korabecna, Marie Sci Rep Article Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-α m RNA expression in monocytes. We performed qPCR to determine relative telomere length (T/S ratios) in plasma, serum and whole blood of 36 volunteers. Using paired samples of plasma and serum and DNase treatment, we analysed the contribution of cfDNA to the co-activation of TNF-α mRNA expression in THP1 monocytic cell line. We found significant differences between paired plasma and serum samples in relative T/S ratios (median 1.38 ± 1.1 vs. 0.86 ± 0.25, respectively) and in total amounts of cfDNA and in estimated total amounts of telomeres which were significantly higher in serum than in plasma. TNF-α mRNA expression in THP1 cells increased significantly after DNase treatment of all samples used for stimulation. The highest TNF-α mRNA expressions were observed after stimulation with DNase treated serum samples. Our results suggest that the different content of telomeric sequences in plasma and serum may contribute to the tuning of immune response. Further studies of this interesting phenomenon are needed. Nature Publishing Group UK 2017-06-01 /pmc/articles/PMC5453964/ /pubmed/28572683 http://dx.doi.org/10.1038/s41598-017-02905-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zinkova, Alzbeta
Brynychova, Iva
Svacina, Alexander
Jirkovska, Marie
Korabecna, Marie
Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title_full Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title_fullStr Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title_full_unstemmed Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title_short Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
title_sort cell-free dna from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453964/
https://www.ncbi.nlm.nih.gov/pubmed/28572683
http://dx.doi.org/10.1038/s41598-017-02905-8
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