Cargando…
Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response
Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-α m RNA expression in monocytes. W...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453964/ https://www.ncbi.nlm.nih.gov/pubmed/28572683 http://dx.doi.org/10.1038/s41598-017-02905-8 |
_version_ | 1783240747391123456 |
---|---|
author | Zinkova, Alzbeta Brynychova, Iva Svacina, Alexander Jirkovska, Marie Korabecna, Marie |
author_facet | Zinkova, Alzbeta Brynychova, Iva Svacina, Alexander Jirkovska, Marie Korabecna, Marie |
author_sort | Zinkova, Alzbeta |
collection | PubMed |
description | Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-α m RNA expression in monocytes. We performed qPCR to determine relative telomere length (T/S ratios) in plasma, serum and whole blood of 36 volunteers. Using paired samples of plasma and serum and DNase treatment, we analysed the contribution of cfDNA to the co-activation of TNF-α mRNA expression in THP1 monocytic cell line. We found significant differences between paired plasma and serum samples in relative T/S ratios (median 1.38 ± 1.1 vs. 0.86 ± 0.25, respectively) and in total amounts of cfDNA and in estimated total amounts of telomeres which were significantly higher in serum than in plasma. TNF-α mRNA expression in THP1 cells increased significantly after DNase treatment of all samples used for stimulation. The highest TNF-α mRNA expressions were observed after stimulation with DNase treated serum samples. Our results suggest that the different content of telomeric sequences in plasma and serum may contribute to the tuning of immune response. Further studies of this interesting phenomenon are needed. |
format | Online Article Text |
id | pubmed-5453964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54539642017-06-02 Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response Zinkova, Alzbeta Brynychova, Iva Svacina, Alexander Jirkovska, Marie Korabecna, Marie Sci Rep Article Circulating cell-free DNA (cfDNA) may be involved in immune response regulation. We studied the variations in abundance of telomeric sequences in plasma and serum in young healthy volunteers and the ability of cfDNA contained in these samples to co-activate the TNF-α m RNA expression in monocytes. We performed qPCR to determine relative telomere length (T/S ratios) in plasma, serum and whole blood of 36 volunteers. Using paired samples of plasma and serum and DNase treatment, we analysed the contribution of cfDNA to the co-activation of TNF-α mRNA expression in THP1 monocytic cell line. We found significant differences between paired plasma and serum samples in relative T/S ratios (median 1.38 ± 1.1 vs. 0.86 ± 0.25, respectively) and in total amounts of cfDNA and in estimated total amounts of telomeres which were significantly higher in serum than in plasma. TNF-α mRNA expression in THP1 cells increased significantly after DNase treatment of all samples used for stimulation. The highest TNF-α mRNA expressions were observed after stimulation with DNase treated serum samples. Our results suggest that the different content of telomeric sequences in plasma and serum may contribute to the tuning of immune response. Further studies of this interesting phenomenon are needed. Nature Publishing Group UK 2017-06-01 /pmc/articles/PMC5453964/ /pubmed/28572683 http://dx.doi.org/10.1038/s41598-017-02905-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zinkova, Alzbeta Brynychova, Iva Svacina, Alexander Jirkovska, Marie Korabecna, Marie Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response |
title | Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response |
title_full | Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response |
title_fullStr | Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response |
title_full_unstemmed | Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response |
title_short | Cell-free DNA from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response |
title_sort | cell-free dna from human plasma and serum differs in content of telomeric sequences and its ability to promote immune response |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5453964/ https://www.ncbi.nlm.nih.gov/pubmed/28572683 http://dx.doi.org/10.1038/s41598-017-02905-8 |
work_keys_str_mv | AT zinkovaalzbeta cellfreednafromhumanplasmaandserumdiffersincontentoftelomericsequencesanditsabilitytopromoteimmuneresponse AT brynychovaiva cellfreednafromhumanplasmaandserumdiffersincontentoftelomericsequencesanditsabilitytopromoteimmuneresponse AT svacinaalexander cellfreednafromhumanplasmaandserumdiffersincontentoftelomericsequencesanditsabilitytopromoteimmuneresponse AT jirkovskamarie cellfreednafromhumanplasmaandserumdiffersincontentoftelomericsequencesanditsabilitytopromoteimmuneresponse AT korabecnamarie cellfreednafromhumanplasmaandserumdiffersincontentoftelomericsequencesanditsabilitytopromoteimmuneresponse |