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Novel approach for accurate tissue-based protein colocalization and proximity microscopy
Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization analyses...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454019/ https://www.ncbi.nlm.nih.gov/pubmed/28572629 http://dx.doi.org/10.1038/s41598-017-02735-8 |
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author | Lutz, Mirjam I. Schwaiger, Carmen Hochreiter, Bernhard Kovacs, Gabor G. Schmid, Johannes A. |
author_facet | Lutz, Mirjam I. Schwaiger, Carmen Hochreiter, Bernhard Kovacs, Gabor G. Schmid, Johannes A. |
author_sort | Lutz, Mirjam I. |
collection | PubMed |
description | Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization analyses together with visualization of physical proximity using fluorescence resonance energy transfer (FRET). To verify our results we used the proximity ligation assay (PLA). With these methods we could demonstrate that distinct neurodegeneration-related proteins either not or only rarely interact in human brain tissue. |
format | Online Article Text |
id | pubmed-5454019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54540192017-06-06 Novel approach for accurate tissue-based protein colocalization and proximity microscopy Lutz, Mirjam I. Schwaiger, Carmen Hochreiter, Bernhard Kovacs, Gabor G. Schmid, Johannes A. Sci Rep Article Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization analyses together with visualization of physical proximity using fluorescence resonance energy transfer (FRET). To verify our results we used the proximity ligation assay (PLA). With these methods we could demonstrate that distinct neurodegeneration-related proteins either not or only rarely interact in human brain tissue. Nature Publishing Group UK 2017-06-01 /pmc/articles/PMC5454019/ /pubmed/28572629 http://dx.doi.org/10.1038/s41598-017-02735-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lutz, Mirjam I. Schwaiger, Carmen Hochreiter, Bernhard Kovacs, Gabor G. Schmid, Johannes A. Novel approach for accurate tissue-based protein colocalization and proximity microscopy |
title | Novel approach for accurate tissue-based protein colocalization and proximity microscopy |
title_full | Novel approach for accurate tissue-based protein colocalization and proximity microscopy |
title_fullStr | Novel approach for accurate tissue-based protein colocalization and proximity microscopy |
title_full_unstemmed | Novel approach for accurate tissue-based protein colocalization and proximity microscopy |
title_short | Novel approach for accurate tissue-based protein colocalization and proximity microscopy |
title_sort | novel approach for accurate tissue-based protein colocalization and proximity microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454019/ https://www.ncbi.nlm.nih.gov/pubmed/28572629 http://dx.doi.org/10.1038/s41598-017-02735-8 |
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