Cargando…

Novel approach for accurate tissue-based protein colocalization and proximity microscopy

Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization analyses...

Descripción completa

Detalles Bibliográficos
Autores principales: Lutz, Mirjam I., Schwaiger, Carmen, Hochreiter, Bernhard, Kovacs, Gabor G., Schmid, Johannes A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454019/
https://www.ncbi.nlm.nih.gov/pubmed/28572629
http://dx.doi.org/10.1038/s41598-017-02735-8
_version_ 1783240761809043456
author Lutz, Mirjam I.
Schwaiger, Carmen
Hochreiter, Bernhard
Kovacs, Gabor G.
Schmid, Johannes A.
author_facet Lutz, Mirjam I.
Schwaiger, Carmen
Hochreiter, Bernhard
Kovacs, Gabor G.
Schmid, Johannes A.
author_sort Lutz, Mirjam I.
collection PubMed
description Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization analyses together with visualization of physical proximity using fluorescence resonance energy transfer (FRET). To verify our results we used the proximity ligation assay (PLA). With these methods we could demonstrate that distinct neurodegeneration-related proteins either not or only rarely interact in human brain tissue.
format Online
Article
Text
id pubmed-5454019
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54540192017-06-06 Novel approach for accurate tissue-based protein colocalization and proximity microscopy Lutz, Mirjam I. Schwaiger, Carmen Hochreiter, Bernhard Kovacs, Gabor G. Schmid, Johannes A. Sci Rep Article Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization analyses together with visualization of physical proximity using fluorescence resonance energy transfer (FRET). To verify our results we used the proximity ligation assay (PLA). With these methods we could demonstrate that distinct neurodegeneration-related proteins either not or only rarely interact in human brain tissue. Nature Publishing Group UK 2017-06-01 /pmc/articles/PMC5454019/ /pubmed/28572629 http://dx.doi.org/10.1038/s41598-017-02735-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lutz, Mirjam I.
Schwaiger, Carmen
Hochreiter, Bernhard
Kovacs, Gabor G.
Schmid, Johannes A.
Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title_full Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title_fullStr Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title_full_unstemmed Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title_short Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title_sort novel approach for accurate tissue-based protein colocalization and proximity microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5454019/
https://www.ncbi.nlm.nih.gov/pubmed/28572629
http://dx.doi.org/10.1038/s41598-017-02735-8
work_keys_str_mv AT lutzmirjami novelapproachforaccuratetissuebasedproteincolocalizationandproximitymicroscopy
AT schwaigercarmen novelapproachforaccuratetissuebasedproteincolocalizationandproximitymicroscopy
AT hochreiterbernhard novelapproachforaccuratetissuebasedproteincolocalizationandproximitymicroscopy
AT kovacsgaborg novelapproachforaccuratetissuebasedproteincolocalizationandproximitymicroscopy
AT schmidjohannesa novelapproachforaccuratetissuebasedproteincolocalizationandproximitymicroscopy