Efficient method for site-directed mutagenesis in large plasmids without subcloning

Commonly used methods for site-directed DNA mutagenesis require copying the entire target plasmid. These methods allow relatively easy modification of DNA sequences in small plasmids but become less efficient and faithful for large plasmids, necessitating full sequence verification. Introduction of...

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Detalles Bibliográficos
Autores principales: Hallak, Louay K., Berger, Kelly, Kaspar, Rita, Kwilas, Anna R., Montanaro, Federica, Peeples, Mark E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456045/
https://www.ncbi.nlm.nih.gov/pubmed/28575024
http://dx.doi.org/10.1371/journal.pone.0177788

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5456045/
https://www.ncbi.nlm.nih.gov/pubmed/28575024
http://dx.doi.org/10.1371/journal.pone.0177788

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