A high-throughput assay for quantitative measurement of PCR errors

The accuracy with which DNA polymerase can replicate a template DNA sequence is an extremely important property that can vary by an order of magnitude from one enzyme to another. The rate of nucleotide misincorporation is shaped by multiple factors, including PCR conditions and proofreading capabili...

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Detalles Bibliográficos
Autores principales: Shagin, Dmitriy A., Shagina, Irina A., Zaretsky, Andrew R., Barsova, Ekaterina V., Kelmanson, Ilya V., Lukyanov, Sergey, Chudakov, Dmitriy M., Shugay, Mikhail
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457411/
https://www.ncbi.nlm.nih.gov/pubmed/28578414
http://dx.doi.org/10.1038/s41598-017-02727-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457411/
https://www.ncbi.nlm.nih.gov/pubmed/28578414
http://dx.doi.org/10.1038/s41598-017-02727-8

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