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Real-time analysis of the binding of fluorescent VEGF(165)a to VEGFR2 in living cells: Effect of receptor tyrosine kinase inhibitors and fate of internalized agonist-receptor complexes
Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis. Here we have used a novel stoichiometric protein-labeling method to generate a fluorescent variant of VEGF (VEGF(165)a-TMR) labeled on a single cysteine within each protomer of the antiparallel VEGF homodimer. VEGF(1...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5457915/ https://www.ncbi.nlm.nih.gov/pubmed/28392095 http://dx.doi.org/10.1016/j.bcp.2017.04.006 |