Hit and go CAS9 delivered through a lentiviral based self-limiting circuit

In vivo application of the CRISPR-Cas9 technology is still limited by unwanted Cas9 genomic cleavages. Long-term expression of Cas9 increases the number of genomic loci non-specifically cleaved by the nuclease. Here we develop a Self-Limiting Cas9 circuit for Enhanced Safety and specificity (SLiCES)...

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Detalles Bibliográficos
Autores principales: Petris, Gianluca, Casini, Antonio, Montagna, Claudia, Lorenzin, Francesca, Prandi, Davide, Romanel, Alessandro, Zasso, Jacopo, Conti, Luciano, Demichelis, Francesca, Cereseto, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458126/
https://www.ncbi.nlm.nih.gov/pubmed/28530235
http://dx.doi.org/10.1038/ncomms15334

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5458126/
https://www.ncbi.nlm.nih.gov/pubmed/28530235
http://dx.doi.org/10.1038/ncomms15334

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