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Use of a restriction enzyme-digested PCR product as substrate for helicase assays
DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not available in all laboratories. Here, a PCR-based method to generate a substrate for a helicase assay is desc...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2005
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC546180/ https://www.ncbi.nlm.nih.gov/pubmed/15653629 http://dx.doi.org/10.1093/nar/gni009 |