Covalent antibody display—an in vitro antibody-DNA library selection system

The endonuclease P2A initiates the DNA replication of the bacteriophage P2 by making a covalent bond with its own phosphate backbone. This enzyme has now been exploited as a new in vitro display tool for antibody fragments. We have constructed genetic fusions of P2A with single-chain antibodies (scF...

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Detalles Bibliográficos
Autores principales: Reiersen, Herald, Løbersli, Inger, Løset, Geir Å., Hvattum, Else, Simonsen, Bjørg, Stacy, John E., McGregor, Duncan, FitzGerald, Kevin, Welschof, Martin, Brekke, Ole H., Marvik, Ole J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2005
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC546181/
https://www.ncbi.nlm.nih.gov/pubmed/15653626
http://dx.doi.org/10.1093/nar/gni010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC546181/
https://www.ncbi.nlm.nih.gov/pubmed/15653626
http://dx.doi.org/10.1093/nar/gni010

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