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Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell
BACKGROUND: Current methods for accurate quantification of nucleic acids typically begin with a template preparation step in which DNA and/or RNA are freed of bound proteins and are then purified. Isolation of RNA is particularly challenging because this molecule is sensitive to elevated temperature...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2005
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC546192/ https://www.ncbi.nlm.nih.gov/pubmed/15649321 http://dx.doi.org/10.1186/1472-6750-5-2 |