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Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues
Our study examined the in vivo chimeric and survival capacities of chimeras created by injecting tetraploid embryonic stem cells (ESCs) expressing green fluorescent protein (GFP) into diploid embryos. At 3.5 days post-coitum (dpc) and 4.5 dpc, the tetraploid ESCs were able to contribute to the inner...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465063/ https://www.ncbi.nlm.nih.gov/pubmed/28596585 http://dx.doi.org/10.1038/s41598-017-02783-0 |
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author | Wen, Bingqiang Li, Ruiqi Cheng, Keren Li, Enhong Zhang, Shaopeng Xiang, Jinzhu Wang, Yanliang Han, Jianyong |
author_facet | Wen, Bingqiang Li, Ruiqi Cheng, Keren Li, Enhong Zhang, Shaopeng Xiang, Jinzhu Wang, Yanliang Han, Jianyong |
author_sort | Wen, Bingqiang |
collection | PubMed |
description | Our study examined the in vivo chimeric and survival capacities of chimeras created by injecting tetraploid embryonic stem cells (ESCs) expressing green fluorescent protein (GFP) into diploid embryos. At 3.5 days post-coitum (dpc) and 4.5 dpc, the tetraploid ESCs were able to contribute to the inner cell mass (ICM) just as diploid ESCs tagged with GFP. At 6.5 dpc, 8.0 dpc and 10.5 dpc, the tetraploid ESCs manifested in the same location as the diploid ESCs. The GFP cells in the extraembryonic tissues and fetuses of tetraploid ESC chimeras were tetraploid as determined by fluorescence activated cell sorting (FACS). Furthermore, tetraploid ESCs contributed to the development of the placenta, embryolemma and umbilical cord at 13.5 dpc and 16.5 dpc; however, very less GFP cells were found in the fetuses of tetraploid ESC chimeras. We further found that the proliferation of tetraploid ESCs was slower than that of diploid ESCs. In addition, the relative mRNA expression in the three germ layers and the trophoblast was abnormal in the EBs of tetraploid ESCs compared with diploid ESCs. In short, slower proliferation and abnormal differentiation potential of tetraploid ESCs might be two of the reasons for their poor survival and chimeric capacities. |
format | Online Article Text |
id | pubmed-5465063 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54650632017-06-14 Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues Wen, Bingqiang Li, Ruiqi Cheng, Keren Li, Enhong Zhang, Shaopeng Xiang, Jinzhu Wang, Yanliang Han, Jianyong Sci Rep Article Our study examined the in vivo chimeric and survival capacities of chimeras created by injecting tetraploid embryonic stem cells (ESCs) expressing green fluorescent protein (GFP) into diploid embryos. At 3.5 days post-coitum (dpc) and 4.5 dpc, the tetraploid ESCs were able to contribute to the inner cell mass (ICM) just as diploid ESCs tagged with GFP. At 6.5 dpc, 8.0 dpc and 10.5 dpc, the tetraploid ESCs manifested in the same location as the diploid ESCs. The GFP cells in the extraembryonic tissues and fetuses of tetraploid ESC chimeras were tetraploid as determined by fluorescence activated cell sorting (FACS). Furthermore, tetraploid ESCs contributed to the development of the placenta, embryolemma and umbilical cord at 13.5 dpc and 16.5 dpc; however, very less GFP cells were found in the fetuses of tetraploid ESC chimeras. We further found that the proliferation of tetraploid ESCs was slower than that of diploid ESCs. In addition, the relative mRNA expression in the three germ layers and the trophoblast was abnormal in the EBs of tetraploid ESCs compared with diploid ESCs. In short, slower proliferation and abnormal differentiation potential of tetraploid ESCs might be two of the reasons for their poor survival and chimeric capacities. Nature Publishing Group UK 2017-06-08 /pmc/articles/PMC5465063/ /pubmed/28596585 http://dx.doi.org/10.1038/s41598-017-02783-0 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wen, Bingqiang Li, Ruiqi Cheng, Keren Li, Enhong Zhang, Shaopeng Xiang, Jinzhu Wang, Yanliang Han, Jianyong Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues |
title | Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues |
title_full | Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues |
title_fullStr | Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues |
title_full_unstemmed | Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues |
title_short | Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues |
title_sort | tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465063/ https://www.ncbi.nlm.nih.gov/pubmed/28596585 http://dx.doi.org/10.1038/s41598-017-02783-0 |
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