Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker

BACKGROUND: A commonly used approach to improve recombinant protein production is to increase the levels of expression by providing extra-copies of a heterologous gene. In Komagataella phaffii (Pichia pastoris) this is usually accomplished by transforming cells with an expression vector carrying a d...

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Detalles Bibliográficos
Autores principales: Betancur, Maritza Ocampo, Reis, Viviane Castelo Branco, Nicola, André Moraes, De Marco, Janice Lisboa, de Moraes, Lídia Maria Pepe, Torres, Fernando Araripe Gonçalves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465527/
https://www.ncbi.nlm.nih.gov/pubmed/28595601
http://dx.doi.org/10.1186/s12934-017-0715-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465527/
https://www.ncbi.nlm.nih.gov/pubmed/28595601
http://dx.doi.org/10.1186/s12934-017-0715-8

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