Cargando…

Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker

BACKGROUND: A commonly used approach to improve recombinant protein production is to increase the levels of expression by providing extra-copies of a heterologous gene. In Komagataella phaffii (Pichia pastoris) this is usually accomplished by transforming cells with an expression vector carrying a d...

Descripción completa

Detalles Bibliográficos
Autores principales:	Betancur, Maritza Ocampo, Reis, Viviane Castelo Branco, Nicola, André Moraes, De Marco, Janice Lisboa, de Moraes, Lídia Maria Pepe, Torres, Fernando Araripe Gonçalves
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2017
Materias:	Technical Notes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5465527/ https://www.ncbi.nlm.nih.gov/pubmed/28595601 http://dx.doi.org/10.1186/s12934-017-0715-8

Ejemplares similares

Construction and characterization of centromeric plasmids for Komagataella phaffii using a color-based plasmid stability assay
por: Piva, Luiza Cesca, et al.
Publicado: (2020)

Use of an on/off tetracycline riboswitch to control protein production in Komagataella phaffii
por: Vieira, Igor Patrick Vasconcelos, et al.
Publicado: (2023)

What makes Komagataella phaffii non-conventional?
por: Ata, Özge, et al.
Publicado: (2021)

Identification and characterization of the Komagataella phaffii mating pheromone genes
por: Heistinger, Lina, et al.
Publicado: (2018)

A subcellular proteome atlas of the yeast Komagataella phaffii
por: Valli, Minoska, et al.
Publicado: (2020)

Komagataella phaffii as Emerging Model Organism in Fundamental Research
por: Bernauer, Lukas, et al.
Publicado: (2021)

Effect of Methionine on Gene Expression in Komagataella phaffii Cells
por: Ianshina, Tatiana, et al.
Publicado: (2023)

Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii
por: Barone, Giovanni Davide, et al.
Publicado: (2023)

Pseudohyphal differentiation in Komagataella phaffii: investigating the FLO gene family
por: De, Sonakshi, et al.
Publicado: (2020)

Histidine is essential for growth of Komagataella phaffii cultured in YPA medium
por: Gupta, Aditi, et al.
Publicado: (2022)

Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization
por: Albacar, Marcel, et al.
Publicado: (2023)

Alternative PCR-Based Approaches for Generation of Komagataella phaffii Strains
por: Makeeva, Anastasiya, et al.
Publicado: (2023)

Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism
por: Xu, Qin, et al.
Publicado: (2019)

Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production
por: Bustos, Cristina, et al.
Publicado: (2022)

Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris)
por: Besleaga, Mihail, et al.
Publicado: (2023)

Correction: Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization
por: Albacar, Marcel, et al.
Publicado: (2023)

Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineered for Lactic Acid Production
por: Melo, Nadiele T. M., et al.
Publicado: (2018)

Process development for the continuous production of heterologous proteins by the industrial yeast, Komagataella phaffii
por: Cankorur‐Cetinkaya, Ayca, et al.
Publicado: (2018)

Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii
por: Alva, Troy R., et al.
Publicado: (2021)

Transcriptome Analysis Unveils the Effects of Proline on Gene Expression in the Yeast Komagataella phaffii
por: Rumyantsev, Andrey, et al.
Publicado: (2021)

Regulation of Peroxisome Homeostasis by Post-Translational Modification in the Methylotrophic Yeast Komagataella phaffii
por: Ohsawa, Shin, et al.
Publicado: (2022)

Heterologous Expression of Extracellular Proteinase pAsPs of Aspergillus pseudotamarii in Komagataella phaffii
por: Zadorozhny, Andrey Valentinovich, et al.
Publicado: (2022)

L-Asparaginase from Penicillium sizovae Produced by a Recombinant Komagataella phaffii Strain
por: Freitas, Marcela, et al.
Publicado: (2022)

Two homologs of the Cat8 transcription factor are involved in the regulation of ethanol utilization in Komagataella phaffii
por: Barbay, Diane, et al.
Publicado: (2021)

Creation of Stable Heterothallic Strains of Komagataella phaffii Enables Dissection of Mating Gene Regulation
por: Heistinger, Lina, et al.
Publicado: (2017)

Role of Dissimilative Pathway of Komagataella phaffii (Pichia pastoris): Formaldehyde Toxicity and Energy Metabolism
por: Berrios, Julio, et al.
Publicado: (2022)

Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
por: Zadorozhny, Andrey Valentinovich, et al.
Publicado: (2022)

Exploring the genetic architecture of protein secretion in Komagataella phaffii (Pichia pastoris) for biotechnology applications
por: Swichkow, Chantle Reiko, et al.
Publicado: (2022)

Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli
por: Pfrimer, Pollyana, et al.
Publicado: (2010)

Xylose Fermentation by Saccharomyces cerevisiae: Challenges and Prospects
por: Moysés, Danuza Nogueira, et al.
Publicado: (2016)

A study on the use of strain-specific and homologous promoters for heterologous expression in industrial Saccharomyces cerevisiae strains
por: de Paiva, Daniel Pereira, et al.
Publicado: (2018)

Centromeres of the Yeast Komagataella phaffii (Pichia pastoris) Have a Simple Inverted-Repeat Structure
por: Coughlan, Aisling Y., et al.
Publicado: (2016)

A single Gal4-like transcription factor activates the Crabtree effect in Komagataella phaffii
por: Ata, Özge, et al.
Publicado: (2018)

Genomic diversity and meiotic recombination among isolates of the biotech yeast Komagataella phaffii (Pichia pastoris)
por: Braun-Galleani, Stephanie, et al.
Publicado: (2019)

Applying the auxin-based degron system for the inducible, reversible and complete protein degradation in Komagataella phaffii
por: Lehmayer, Leonie, et al.
Publicado: (2022)

Coordinate regulation of methanol utilization pathway genes of Komagataella phaffii by transcription factors and chromatin modifiers
por: Gupta, Aditi, et al.
Publicado: (2022)

Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid
por: Àvila-Cabré, Sílvia, et al.
Publicado: (2023)

Metabolic modeling to identify engineering targets for Komagataella phaffii: The effect of biomass composition on gene target identification
por: Cankorur‐Cetinkaya, Ayca, et al.
Publicado: (2017)

Depletion of Boric Acid and Cobalt from Cultivation Media: Impact on Recombinant Protein Production with Komagataella phaffii
por: Pekarsky, Alexander, et al.
Publicado: (2020)

Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album
por: Beklemishev, A.B., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_oklmv7vrglg015a03pogv17csi