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Functional mapping of yeast genomes by saturated transposition

Yeast is a powerful model for systems genetics. We present a versatile, time- and labor-efficient method to functionally explore the Saccharomyces cerevisiae genome using saturated transposon mutagenesis coupled to high-throughput sequencing. SAturated Transposon Analysis in Yeast (SATAY) allows one...

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Detalles Bibliográficos
Autores principales: Michel, Agnès H, Hatakeyama, Riko, Kimmig, Philipp, Arter, Meret, Peter, Matthias, Matos, Joao, De Virgilio, Claudio, Kornmann, Benoît
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466422/
https://www.ncbi.nlm.nih.gov/pubmed/28481201
http://dx.doi.org/10.7554/eLife.23570