Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase

The hydantoinase process is applied for the industrial synthesis of optically pure amino acids via whole cell biocatalysis, providing a simple and well-established method to obtain the catalyst. Nevertheless, whole cell approaches also bear disadvantages like intracellular degradation reactions, tra...

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Detalles Bibliográficos
Autores principales: Slomka, Christin, Späth, Georg Paris, Lemke, Phillip, Skoupi, Marc, Niemeyer, Christof M., Syldatk, Christoph, Rudat, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466576/
https://www.ncbi.nlm.nih.gov/pubmed/28605882
http://dx.doi.org/10.1186/s13568-017-0420-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466576/
https://www.ncbi.nlm.nih.gov/pubmed/28605882
http://dx.doi.org/10.1186/s13568-017-0420-3

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