Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein and/or -ligand interactions as well as GPCR oligomerisation. However the utility of BRET is limited by the requirement tha...

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Detalles Bibliográficos
Autores principales: White, Carl W., Vanyai, Hannah K., See, Heng B., Johnstone, Elizabeth K. M., Pfleger, Kevin D. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466623/
https://www.ncbi.nlm.nih.gov/pubmed/28600500
http://dx.doi.org/10.1038/s41598-017-03486-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466623/
https://www.ncbi.nlm.nih.gov/pubmed/28600500
http://dx.doi.org/10.1038/s41598-017-03486-2

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