Cargando…
Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time
Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein and/or -ligand interactions as well as GPCR oligomerisation. However the utility of BRET is limited by the requirement tha...
| Autores principales: | , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2017
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5466623/ https://www.ncbi.nlm.nih.gov/pubmed/28600500 http://dx.doi.org/10.1038/s41598-017-03486-2 |