Optimized Method for Robust Transcriptome Profiling of Minute Tissues Using Laser Capture Microdissection and Low-Input RNA-Seq

Obtaining high quality RNA from complex biological tissues, such as the brain, is needed for establishing high-fidelity cell-type specific transcriptomes. Although combining genetic labeling techniques with laser capture microdissection (LCM) is generally sufficient, concerns over RNA degradation an...

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Detalles Bibliográficos
Autores principales: Farris, Shannon, Wang, Yu, Ward, James M., Dudek, Serena M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468370/
https://www.ncbi.nlm.nih.gov/pubmed/28659759
http://dx.doi.org/10.3389/fnmol.2017.00185

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5468370/
https://www.ncbi.nlm.nih.gov/pubmed/28659759
http://dx.doi.org/10.3389/fnmol.2017.00185

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