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Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage

Chromatin exhibits increased mobility on DNA damage, but the biophysical basis for this behavior remains unknown. To explore the mechanisms that drive DNA damage–induced chromosome mobility, we use single-particle tracking of tagged chromosomal loci during interphase in live yeast cells together wit...

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Detalles Bibliográficos
Autores principales: Lawrimore, Josh, Barry, Timothy M., Barry, Raymond M., York, Alyssa C., Friedman, Brandon, Cook, Diana M., Akialis, Kristen, Tyler, Jolien, Vasquez, Paula, Yeh, Elaine, Bloom, Kerry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469612/
https://www.ncbi.nlm.nih.gov/pubmed/28450453
http://dx.doi.org/10.1091/mbc.E16-12-0846
Descripción
Sumario:Chromatin exhibits increased mobility on DNA damage, but the biophysical basis for this behavior remains unknown. To explore the mechanisms that drive DNA damage–induced chromosome mobility, we use single-particle tracking of tagged chromosomal loci during interphase in live yeast cells together with polymer models of chromatin chains. Telomeres become mobilized from sites on the nuclear envelope and the pericentromere expands after exposure to DNA-damaging agents. The magnitude of chromatin mobility induced by a single double-strand break requires active microtubule function. These findings reveal how relaxation of external tethers to the nuclear envelope and internal chromatin–chromatin tethers, together with microtubule dynamics, can mobilize the genome in response to DNA damage.