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Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage
Chromatin exhibits increased mobility on DNA damage, but the biophysical basis for this behavior remains unknown. To explore the mechanisms that drive DNA damage–induced chromosome mobility, we use single-particle tracking of tagged chromosomal loci during interphase in live yeast cells together wit...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469612/ https://www.ncbi.nlm.nih.gov/pubmed/28450453 http://dx.doi.org/10.1091/mbc.E16-12-0846 |
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author | Lawrimore, Josh Barry, Timothy M. Barry, Raymond M. York, Alyssa C. Friedman, Brandon Cook, Diana M. Akialis, Kristen Tyler, Jolien Vasquez, Paula Yeh, Elaine Bloom, Kerry |
author_facet | Lawrimore, Josh Barry, Timothy M. Barry, Raymond M. York, Alyssa C. Friedman, Brandon Cook, Diana M. Akialis, Kristen Tyler, Jolien Vasquez, Paula Yeh, Elaine Bloom, Kerry |
author_sort | Lawrimore, Josh |
collection | PubMed |
description | Chromatin exhibits increased mobility on DNA damage, but the biophysical basis for this behavior remains unknown. To explore the mechanisms that drive DNA damage–induced chromosome mobility, we use single-particle tracking of tagged chromosomal loci during interphase in live yeast cells together with polymer models of chromatin chains. Telomeres become mobilized from sites on the nuclear envelope and the pericentromere expands after exposure to DNA-damaging agents. The magnitude of chromatin mobility induced by a single double-strand break requires active microtubule function. These findings reveal how relaxation of external tethers to the nuclear envelope and internal chromatin–chromatin tethers, together with microtubule dynamics, can mobilize the genome in response to DNA damage. |
format | Online Article Text |
id | pubmed-5469612 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-54696122017-08-30 Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage Lawrimore, Josh Barry, Timothy M. Barry, Raymond M. York, Alyssa C. Friedman, Brandon Cook, Diana M. Akialis, Kristen Tyler, Jolien Vasquez, Paula Yeh, Elaine Bloom, Kerry Mol Biol Cell Articles Chromatin exhibits increased mobility on DNA damage, but the biophysical basis for this behavior remains unknown. To explore the mechanisms that drive DNA damage–induced chromosome mobility, we use single-particle tracking of tagged chromosomal loci during interphase in live yeast cells together with polymer models of chromatin chains. Telomeres become mobilized from sites on the nuclear envelope and the pericentromere expands after exposure to DNA-damaging agents. The magnitude of chromatin mobility induced by a single double-strand break requires active microtubule function. These findings reveal how relaxation of external tethers to the nuclear envelope and internal chromatin–chromatin tethers, together with microtubule dynamics, can mobilize the genome in response to DNA damage. The American Society for Cell Biology 2017-06-15 /pmc/articles/PMC5469612/ /pubmed/28450453 http://dx.doi.org/10.1091/mbc.E16-12-0846 Text en © 2017 Lawrimore et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Lawrimore, Josh Barry, Timothy M. Barry, Raymond M. York, Alyssa C. Friedman, Brandon Cook, Diana M. Akialis, Kristen Tyler, Jolien Vasquez, Paula Yeh, Elaine Bloom, Kerry Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage |
title | Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage |
title_full | Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage |
title_fullStr | Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage |
title_full_unstemmed | Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage |
title_short | Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage |
title_sort | microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to dna damage |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469612/ https://www.ncbi.nlm.nih.gov/pubmed/28450453 http://dx.doi.org/10.1091/mbc.E16-12-0846 |
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