Cargando…

Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage

Chromatin exhibits increased mobility on DNA damage, but the biophysical basis for this behavior remains unknown. To explore the mechanisms that drive DNA damage–induced chromosome mobility, we use single-particle tracking of tagged chromosomal loci during interphase in live yeast cells together wit...

Descripción completa

Detalles Bibliográficos
Autores principales: Lawrimore, Josh, Barry, Timothy M., Barry, Raymond M., York, Alyssa C., Friedman, Brandon, Cook, Diana M., Akialis, Kristen, Tyler, Jolien, Vasquez, Paula, Yeh, Elaine, Bloom, Kerry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469612/
https://www.ncbi.nlm.nih.gov/pubmed/28450453
http://dx.doi.org/10.1091/mbc.E16-12-0846
_version_ 1783243610134675456
author Lawrimore, Josh
Barry, Timothy M.
Barry, Raymond M.
York, Alyssa C.
Friedman, Brandon
Cook, Diana M.
Akialis, Kristen
Tyler, Jolien
Vasquez, Paula
Yeh, Elaine
Bloom, Kerry
author_facet Lawrimore, Josh
Barry, Timothy M.
Barry, Raymond M.
York, Alyssa C.
Friedman, Brandon
Cook, Diana M.
Akialis, Kristen
Tyler, Jolien
Vasquez, Paula
Yeh, Elaine
Bloom, Kerry
author_sort Lawrimore, Josh
collection PubMed
description Chromatin exhibits increased mobility on DNA damage, but the biophysical basis for this behavior remains unknown. To explore the mechanisms that drive DNA damage–induced chromosome mobility, we use single-particle tracking of tagged chromosomal loci during interphase in live yeast cells together with polymer models of chromatin chains. Telomeres become mobilized from sites on the nuclear envelope and the pericentromere expands after exposure to DNA-damaging agents. The magnitude of chromatin mobility induced by a single double-strand break requires active microtubule function. These findings reveal how relaxation of external tethers to the nuclear envelope and internal chromatin–chromatin tethers, together with microtubule dynamics, can mobilize the genome in response to DNA damage.
format Online
Article
Text
id pubmed-5469612
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-54696122017-08-30 Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage Lawrimore, Josh Barry, Timothy M. Barry, Raymond M. York, Alyssa C. Friedman, Brandon Cook, Diana M. Akialis, Kristen Tyler, Jolien Vasquez, Paula Yeh, Elaine Bloom, Kerry Mol Biol Cell Articles Chromatin exhibits increased mobility on DNA damage, but the biophysical basis for this behavior remains unknown. To explore the mechanisms that drive DNA damage–induced chromosome mobility, we use single-particle tracking of tagged chromosomal loci during interphase in live yeast cells together with polymer models of chromatin chains. Telomeres become mobilized from sites on the nuclear envelope and the pericentromere expands after exposure to DNA-damaging agents. The magnitude of chromatin mobility induced by a single double-strand break requires active microtubule function. These findings reveal how relaxation of external tethers to the nuclear envelope and internal chromatin–chromatin tethers, together with microtubule dynamics, can mobilize the genome in response to DNA damage. The American Society for Cell Biology 2017-06-15 /pmc/articles/PMC5469612/ /pubmed/28450453 http://dx.doi.org/10.1091/mbc.E16-12-0846 Text en © 2017 Lawrimore et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Lawrimore, Josh
Barry, Timothy M.
Barry, Raymond M.
York, Alyssa C.
Friedman, Brandon
Cook, Diana M.
Akialis, Kristen
Tyler, Jolien
Vasquez, Paula
Yeh, Elaine
Bloom, Kerry
Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage
title Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage
title_full Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage
title_fullStr Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage
title_full_unstemmed Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage
title_short Microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to DNA damage
title_sort microtubule dynamics drive enhanced chromatin motion and mobilize telomeres in response to dna damage
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469612/
https://www.ncbi.nlm.nih.gov/pubmed/28450453
http://dx.doi.org/10.1091/mbc.E16-12-0846
work_keys_str_mv AT lawrimorejosh microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage
AT barrytimothym microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage
AT barryraymondm microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage
AT yorkalyssac microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage
AT friedmanbrandon microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage
AT cookdianam microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage
AT akialiskristen microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage
AT tylerjolien microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage
AT vasquezpaula microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage
AT yehelaine microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage
AT bloomkerry microtubuledynamicsdriveenhancedchromatinmotionandmobilizetelomeresinresponsetodnadamage