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Coupling neutron reflectivity with cell-free protein synthesis to probe membrane protein structure in supported bilayers
The structure of the p7 viroporin, an oligomeric membrane protein ion channel involved in the assembly and release of the hepatitis C virus, was determined from proteins expressed and inserted directly into supported model lipid membranes using cell-free protein expression. Cell-free protein express...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469739/ https://www.ncbi.nlm.nih.gov/pubmed/28611396 http://dx.doi.org/10.1038/s41598-017-03472-8 |