Cargando…
Coupling neutron reflectivity with cell-free protein synthesis to probe membrane protein structure in supported bilayers
The structure of the p7 viroporin, an oligomeric membrane protein ion channel involved in the assembly and release of the hepatitis C virus, was determined from proteins expressed and inserted directly into supported model lipid membranes using cell-free protein expression. Cell-free protein express...
Autores principales: | Soranzo, Thomas, Martin, Donald K., Lenormand, Jean-Luc, Watkins, Erik B. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469739/ https://www.ncbi.nlm.nih.gov/pubmed/28611396 http://dx.doi.org/10.1038/s41598-017-03472-8 |
Ejemplares similares
-
Toward Electrophoretic Separation of Membrane Proteins
in Supported n-Bilayers
por: Harb, Frédéric F., et al.
Publicado: (2020) -
G-Protein Coupled Receptor Protein Synthesis on a Lipid Bilayer Using a Reconstituted Cell-Free Protein Synthesis System
por: Gessesse, Belay, et al.
Publicado: (2018) -
Nanodisc Films for Membrane Protein Studies by Neutron
Reflection: Effect of the Protein Scaffold Choice
por: Bertram, Nicolas, et al.
Publicado: (2015) -
Cell-free expression of the outer membrane protein OprF of Pseudomonas aeruginosa for vaccine purposes
por: Mayeux, Géraldine, et al.
Publicado: (2021) -
Small-Angle Neutron Scattering for Studying Lipid Bilayer Membranes
por: Heller, William T.
Publicado: (2022)