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Targeted next-generation sequencing of the 16S-23S rRNA region for culture-independent bacterial identification - increased discrimination of closely related species

The aim of this study was to develop an easy-to-use culture-free diagnostic method based on next generation sequencing (NGS) of PCR amplification products encompassing whole 16S-23S rRNA region to improve the resolution of bacterial species identification. To determine the resolution of the new meth...

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Detalles Bibliográficos
Autores principales: Sabat, Artur J., van Zanten, Evert, Akkerboom, Viktoria, Wisselink, Guido, van Slochteren, Kees, de Boer, Richard F., Hendrix, Ron, Friedrich, Alexander W., Rossen, John W. A., Kooistra-Smid, Anna M. D. (Mirjam)
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469791/
https://www.ncbi.nlm.nih.gov/pubmed/28611406
http://dx.doi.org/10.1038/s41598-017-03458-6