Unexpected consequences: exon skipping caused by CRISPR-generated mutations

A new study finds that splicing disruption is a frequent consequence of mutations generated by CRISPR/Cas9 gene-editing technology, and alleles designed to be null can express aberrant proteins. This new information allows enhanced quality control procedures to select the best mutant alleles generat...

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Detalles Bibliográficos
Autores principales: Sharpe, Joshua J., Cooper, Thomas A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research Highlight
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470267/
https://www.ncbi.nlm.nih.gov/pubmed/28615035
http://dx.doi.org/10.1186/s13059-017-1240-0
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author Sharpe, Joshua J.
Cooper, Thomas A.
author_facet Sharpe, Joshua J.
Cooper, Thomas A.
author_sort Sharpe, Joshua J.
collection PubMed
description A new study finds that splicing disruption is a frequent consequence of mutations generated by CRISPR/Cas9 gene-editing technology, and alleles designed to be null can express aberrant proteins. This new information allows enhanced quality control procedures to select the best mutant alleles generated by CRISPR/Cas9. Please see related Method article: https://www.doi.org/10.1186/s13059-017-1237-8
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spelling pubmed-54702672017-06-19 Unexpected consequences: exon skipping caused by CRISPR-generated mutations Sharpe, Joshua J. Cooper, Thomas A. Genome Biol Research Highlight A new study finds that splicing disruption is a frequent consequence of mutations generated by CRISPR/Cas9 gene-editing technology, and alleles designed to be null can express aberrant proteins. This new information allows enhanced quality control procedures to select the best mutant alleles generated by CRISPR/Cas9. Please see related Method article: https://www.doi.org/10.1186/s13059-017-1237-8 BioMed Central 2017-06-14 /pmc/articles/PMC5470267/ /pubmed/28615035 http://dx.doi.org/10.1186/s13059-017-1240-0 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Highlight
Sharpe, Joshua J.
Cooper, Thomas A.
Unexpected consequences: exon skipping caused by CRISPR-generated mutations
title Unexpected consequences: exon skipping caused by CRISPR-generated mutations
title_full Unexpected consequences: exon skipping caused by CRISPR-generated mutations
title_fullStr Unexpected consequences: exon skipping caused by CRISPR-generated mutations
title_full_unstemmed Unexpected consequences: exon skipping caused by CRISPR-generated mutations
title_short Unexpected consequences: exon skipping caused by CRISPR-generated mutations
title_sort unexpected consequences: exon skipping caused by crispr-generated mutations
topic Research Highlight
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470267/
https://www.ncbi.nlm.nih.gov/pubmed/28615035
http://dx.doi.org/10.1186/s13059-017-1240-0
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AT cooperthomasa unexpectedconsequencesexonskippingcausedbycrisprgeneratedmutations

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